Participants included 278 children with typical development, 288 children with autistic disorder, and 141 children with ASD, ages 24 to 60 months. Via telephone interviews, mothers were questioned retroactively about their consumption of vitamin supplements during the three months prior to conception and throughout the gestational period. Furthermore, blood samples were collected from the families of 232 participants with typical development and 238 participants with autistic disorder. Maternal, paternal, and child blood samples were tested for various one-carbon metabolism gene variations, which have been found in previous research studies to be associated with ASD.
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